感染、炎症、修复2012，Vol.13Issue(3)：151-154,封3,5.
高原严重烫伤大鼠延迟复苏肾组织细胞凋亡与c-fos蛋白表达的变化
Study on the changes in apoptosis and expression of c-fos protein in renal cells after delayed fluid resuscitation in rats with severe burn in regions of high altitude
摘要
Abstract
Objective: To observe the changes in c-fos protein expression in kidney tissue of rats after severe burn with delayed fluid resuscitation (DFR) in areas of different altitude,and explore the relationship between its expression and cell apoptosis. Methods: The experiments were performed at altitudes of 1 517 m and 3 848 m. At the area of the respective altitude, 120 Wistar rats were randomly divided into 3 groups: ① Immediate fluid resuscitation (IFR) group (n2 = 60) : rats were exposed to 30% total body surface area thermal injury (90 ℃ hot water,20 seconds) followed by peritoneal injection of normal saline (4 ml/kg) immediately after burn. ②DFR group (n = 50): after the same injury in IFR group, normal saline was instead injected peritoneally 6 hours after burn. ③Sham-injured group(n = 10) : the rats were sham-injured (37 ℃ water,20 seconds) without fluid injection. The apoptosis of renal cells was assessed by TUNEL,the expression of c-fos protein in kidney tissue was assessed by immunohistochemistry staining and image analysis system 1,6,12,24,72 and 168 hours after burn,and 1 hour after sham injury. The apoptosis index was calculated. Results: At the same altitude,the renal cells apoptosis index and the expression of c-fos protein in DFR group were enhanced compared to those of the IFR group, and they were higher than those of the sham-injured group (P<0. 001). The parameters mentioned-above were higher in high attitude groups compared with those in low attitude groups(P<0. 001). The cells apoptosis was positively correlated to the expression of c-fos protein(r = 0. 968,P<0. 001). Conclusions: Severe burn at the area of high attitude results in high expression of c-fos and an increase in apotosis of kidney cells,and the expression of c-fos is correlated with renal cell apoptosis. Delayed resuscitation could aggravate the apoptosis of kidney cells.关键词
烫伤/延迟复苏/高原/细胞凋亡/c-fos/蛋白Key words
Burns Delayed resuscitation Altitude Apoptosis c-fos引用本文复制引用
姜疆,刘毅,张诚,张绪生,张鲜英,肖斌,张斌..高原严重烫伤大鼠延迟复苏肾组织细胞凋亡与c-fos蛋白表达的变化[J].感染、炎症、修复,2012,13(3):151-154,封3,5.基金项目
全军医学科研"十一五"计划课题(06G030) （06G030）
