果树学报2012，Vol.29Issue(5)：722-728,7.
新疆野苹果分离群体的构建和评价
Construction and evaluation of the segregation population in Malus sieversii
摘要
Abstract
[Objective]The objective of the study is to construct the optimal segregation population for constructing the genetic linkage map in Malus sieversii. [Method]Using red ‘Fuji＇ ×Xinjianghongrou apple as experimental material, a segregation population for M. sieversii had been established by artificial pollination, and the polymorphism between parents, hybrid purity and genetic structure among all progenies had been detected by microsatellite marker. [Result]The results indicated that there was larger genetic variation between red＇Fuji＇ and Xinjianghongrou apple. Excluding 15 undefined progenies, 110 progenies of the segregation population were used to construct the genetic linkage map in M. sieversii. There was no repetition phenomenon among all progenies. 232 polymorphic fragments were detected by 64 SSR primers, among which 191 （82.3%） segregated in the expected Mendelian 1：1 ratio （P〈 0.01）. [Conclusion] The studies suggested that the segregation population of red ‘Fuji＇ ×Xinjianghongrou apple was optimal to construct the genetic linkage map in M. sieversii.关键词
新疆野苹果/SSR/分离群体Key words
Malus sieversii/SSR/Segregation population分类
农业科技引用本文复制引用
刘遵春,苗卫东,刘大亮,陈学森..新疆野苹果分离群体的构建和评价[J].果树学报,2012,29(5):722-728,7.基金项目
国家自然科学基金项目 （）
山东省农业良种工程项目 （）
