果树学报2012，Vol.29Issue(5)：825-829,F0003,6.
抗病抗寒无核葡萄胚挽救研究
Embryo rescue of seedless grape with disease and cold resistance
摘要
Abstract
[Objective]The study is to breed new seedless grape varieties with disease and cold resistance. [Method]Hybridization was carried using two stenospemocarpic seedless grape cuhivars （Vitis vitrifera） ‘Sultanina Rose＇ and ‘Perlette＇ as female parents, two clones of Chinese wild Vitis amurensis ‘Hei- longjiang Seedling＇ and ‘ Shuangyou＇, as well as two interspecific hybrids ＇ Beichun＇ （Muscat Hamburg × V. amurensis） and00-1-10 （Muscat Hamburg × V. amurensis clone Heilongjiang Seedling） as male parents. On 51 days after pollination, ovules were cultivated on ER and MM4 medium by adopting embryo rescue technique, sixty days later germinated on WPM ＋ BA 0.2 mg·L^（-1） medium： [Result]The results showed that two female cuhivars adapted to different medium. The proper medium for ‘ Perlette＇ was ER, for ; ‘Suhanina Rose＇ was MM4. The development rate and germination rate of two female parents were insignificant, and Perlette was little better than Suhanina Rose. However, four male parents had a greater impact on embryo rescue, two interspecific hybrids ‘00-1-10＇ and ‘Beichun＇ were more suitable for male parents than Chinese wild Vitis amurensis Heilongjiang Seedling and Shuangyou. Finally, fifty lines which belonged to new grape germplasm were obtained in this study. [Conclusion]The studies suggested that different seedless grape genotypes need different basic culture media, and interspecific hybrids from V. vinifera and V. amurensis are more suitable for male parents than Chinese wild Vitis amurensis in embryo rescue.关键词
无核葡萄/抗病/抗寒/胚挽救Key words
Seedless grape/Disease resistance/Cold resistance/Embryo rescue分类
农业科技引用本文复制引用
牛茹萱,张剑侠,王跃进,翟焕,赵凯..抗病抗寒无核葡萄胚挽救研究[J].果树学报,2012,29(5):825-829,F0003,6.基金项目
国家现代农业产业技术体系建设专项基金 （）
陕西省科技攻关项目 （）
