果树学报2012，Vol.29Issue(6)：990-996,7.
中国野生毛葡萄病原菌诱导响应VqCOI1基因的克隆及表达分析
Cloning and expression analysis of a pathogen-responsive VqCOI1 gene in Chinese wild Vitis quinquangularis
摘要
Abstract
【Objective】 The objective of the study is to verify the role of COI1 gene in grape disease resistance.【Method】Based on a EST sequence of COI1 gene in the subtractive suppression hybridization(SSH) cDNA library from young leaves of Chinese wild Vitis quinquangularis clone 'shang-24' inoculated with Uncinula necator(Schw.) Burr,we isolated the cDNA sequence of that COI1 gene using the RT-PCR technology,which was designated as VqCOI1.Then we studied its expression patterns by Semi-Quantitative RT-PCR and Quantitative RT-PCR,on the purpose of verifying its roles in grape disease resistance.【Result】The sequence analysis indicated that the length of VqCOI1 was 2 455 bp,which encodes 598 amino acids,with a predicted molecular weight of 67.87 ku and the isoelectric point of 5.85.Multiple Alignment of deduced amino acid sequence showed that it has the highest sequence similarity,approximately 79% similarity with other COI1 in Glycine max(GenBank acc.no: AAZ66745.1).The qRT-PCR showed that the expression of VqCOI1 was induced and increased in the early stages when inoculated with U.nector in the resistant line 'Shang-24',which suggested that VqCOI1 might be involved in pathogen-induced JA signal regulatory pathways.VqCOI1 was also regulated by Ethylene(Eth),Methyl jasmonate(MeJA) and Salicylic acid(SA),and these signaling molecules may cross-regulate the expression of this gene.VqCOI1 was also expressed differentially in leaves,stems,flowers,pericarps and tendrils.【Conclusion】 These results revealed that VqCOI1 was a pathogen-responsive gene and it was regulated by certain biotic stress-related hormones,indicating it may play an important role in grape pathogen resistance.关键词
中国野生葡萄/VqCOI1基因/克隆/表达分析Key words
Vitis quinquangularis/Clone/VqCOI1 gene/Gxpression analysis分类
农业科技引用本文复制引用
牛姣,周存田,王西平..中国野生毛葡萄病原菌诱导响应VqCOI1基因的克隆及表达分析[J].果树学报,2012,29(6):990-996,7.基金项目
国家自然科学基金 （）
