果树学报2012，Vol.29Issue(6)：1092-1096,I0002,6.
杧果畸形病病原菌的营养体亲和性研究
Vegetative compatibility of Fusarium mangiferae strains from mango malformation disease
摘要
Abstract
【Objective】In order to explicit the vegetative compatibility groups(VGC) of Fusarium mangiferae,【Method】the nitrate-nonutilizing(nit) mutant complementation technique was used to explore the population structure of the fungus.【Result】The 38 strains of F.mangiferae were isolated from different mango cultivars from Panzhihua,Sichuan and Huaping,Yunan Providence in China.The tested single-conidium strains were then transplanted on KClO3-containing potato sucrose agar(KPS) plates to mutagenize nit mutants and 455 nit mutants were recovered from the isolates by transferring chlorate-resistant sectors from KPS plates to minimal medium(MM).The four mutant types were identified as nit A,nit B,nit C and nit D based on the growing situation of the mutants on three kinds of different nitrogen media,MM,nitrite medium(NM) and hypoxanthine medium(HM).Among the four types,nit A was the most common one,with the frequency of 78.02%.Next were nit B and nit C,with the frequency of 7.91% and 13.63% respectively.The least one was nit D,0.44%.The nit mutants then were paired on MM plates to test the vegetative compatibility of all isolates,and totally five different VCGs were identified.38 strains were assigned to 5 VCG,30 strains in VCG1,3 strains in VCG2,VCG3 and VCG4 contains 2 strains,respectively,strain MG33 formed separate VCG5.【Conclusion】The correlation between vegetative compatibility of F.mangiferae and mango cultivars or geographical origin was insignificant.关键词
杧果畸形病/nit突变体/抗氯酸盐突变体/营养亲和群Key words
Mango malformation disease/nit mutants/Chlorate resistant mutants/Vegetative compatibility groups分类
农业科技引用本文复制引用
柳凤,韦继光,詹儒林,常金梅..杧果畸形病病原菌的营养体亲和性研究[J].果树学报,2012,29(6):1092-1096,I0002,6.基金项目
中央级非盈利性研究机构基本科研业务费研究专项 （）
海南省自然科学基金 （）
