果树学报2012,Vol.29Issue(6):1097-1102,I0001,7.
绿色木霉菌发酵液对杧果炭疽菌的生长抑制及细胞损伤作用
Effect of Trichoderma uiride fermentation broth on Colletotrichum gloeosporioides growth inhibition and cell damage
摘要
Abstract
【Objective】The mechanism of Trichoderma viride fermentation broth against Colletotrichum gloeosporioides Penz was studied.【Method】C.gloeosporioides Penz,was treated with T.viride fermentation broth,then the hypha growth,spore germination,absorption of phosphoru and membrane permeability of C.gloeosporioides Penz was determined.【Result】The hypha treated with T.viride fermentation broth withered and deformed seriously,and the spore germination in the test group was 86.40% lower than in the control group.Besides,the absorption of phosphorus was almost stopped,both nucleic acid and protein outflowed seriously after the application of T.viride fermentation broth;the contents of reducing sugar and soluble protein in test group were significantly lower than in control group.【Conclusion】T.viride fermentation broth could undermine the integrity of C.gloeosporioides Penz's cell,and suppress the growth of C.gloeosporioides Penz.关键词
绿色木霉菌/发酵液/杧果炭疽菌/生长抑制/细胞损伤Key words
Trichoderma viride/Fermentation broth/Colletotrichum gloeosporioides Penz/Growth inhibition/Cell damage分类
农业科技引用本文复制引用
刘淑宇,于新,陈发河,杨鹏斌,杨静,黄晓敏..绿色木霉菌发酵液对杧果炭疽菌的生长抑制及细胞损伤作用[J].果树学报,2012,29(6):1097-1102,I0001,7.基金项目
国家自然科学基金 ()