国际妇产科学杂志2012，Vol.39Issue(5)：518-520,3.
基因芯片筛选上调 miRNA-205表达后HeLa细胞相关基因
Analysis of Gene Expression Profile in HeLa Cells Upregulated Expression of MicroRNA-205 by cDNA Microarray
摘要
Abstract
Objective:To identify a set of genes related to upregulated expression of raiR-205 in HeLa cells and to investigate their function,and to discuss the pathogenesis of cervical cancer. Methods:The HeLa cells upregulated expression of miR-205 and normal HeLa cells were analyzed with 23 232 human genes of cDNA microarrays. Results: A total of 172 differential genes expressed in the HeLa cells upregulated expression of miR-205 were screened out,of which,34 were up regulated and 138 were down regulated. Conclusions:The cDNA microarray is a powerful method to identify the gene expression profile. Cervical cancer induced by upregulated expression of miR-205 is a result of coeffecting by polygene and multiple factors.关键词
宫颈肿瘤/HeLa细胞/芯片分析技术/微RNAs/基因表达Key words
Uterine cervical neoplasms/Hela cells/Microchip analytical procedures/MicroRNAs/Gene expression引用本文复制引用
韦丽娅,姚婷婷,陈庆瑜,沈青丽,林仲秋..基因芯片筛选上调 miRNA-205表达后HeLa细胞相关基因[J].国际妇产科学杂志,2012,39(5):518-520,3.基金项目
国家自然科学基金(81101379) （81101379）
广东省自然科学基金(S2011040004639) （S2011040004639）
广东省医学科研基金(B2011088) （B2011088）
