华北农学报2012，Vol.27Issue(4)：60-64,5.
7种作物叶绿体基因的密码子偏好性及聚类分析
Codon Usage Bias and Cluster Analysis on Chloroplastic Genes from Seven Crop Species
摘要
Abstract
The analysis of coded characters of crop chloroplast genes is benefit to the genetic engineering design of chloroplast, and the efficient and stable expression of exotic genes in recipient crops. In this study, the codon usage bias of genes encoded by chloroplast DNA in 7 field crops were analyzed by means of several softwares. Results showed that there was little difference among the characters of chloroplast-coded genes among the 7 crops, which held a higher content of A and lower content of G and C. However, there were significant differences in the base composition of the third codon. The codons in the 3 dicotyledon crops were preferred to end with C,and second with T,while the other 4 gramineous crops preferred the codons ended with prior T,and second with C. There were 26 preferable codons used in the 4 gramineous crops and 25 in the 3 dicotyledon plants, among which 22 codons were totally same between the 2 kinds of crops. The cluster analysis based on RSCU value showed that the codon usage bias in the genes encoded by chloroplast was related to the plant species, indicating that the cluster analysis of RSCU in chloroplast genes could be used as a complementary method for the systematic developmental analysis.关键词
作物/叶绿体基因/密码子偏好性/聚类分析Key words
Crop/ Chloroplastic gene/ Codon usage bias/ Cluster analysis分类
生物科学引用本文复制引用
晁岳恩,常阳,王美芳,何盛莲,赵献林,雷振生..7种作物叶绿体基因的密码子偏好性及聚类分析[J].华北农学报,2012,27(4):60-64,5.基金项目
农业部转基因生物新品种培育科技重大专项(2009ZX08002-002B （）
2009ZX08002-014B) （）
