华北农学报2012，Vol.27Issue(4)：123-129,7.
鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究
Differentiation of Umbilical Cord Mesenchymal Stem Cells (UCMSCs) Derived from Chicken into Isletβ-cells in vitro
摘要
Abstract
The aim was to study on the biological characteristics of chicken UCMSCs by isolating culture in vitro. UCMSCs was isolated from umbilical cord of 11 - 15 day-old chicken embryos . The suitable culture medium for UCMSCs was L-DMEM. Then the morphology and the growing proliferation condition and the differentiation potential in vitro of the chicken UCMSCs were observed. The results showed that Chicken UCMSCs can be cultured in vitro and have been passed to 30 generation. The UCMSCs expressed CD29,CD44 and CD71 .results displayed that the UCMSCs had the differentiation potential to isletfj-cells. UCMSCs from chicken can proliferate rapidly in vitro and be induced to isletp-cells.关键词
鸡/脐带间充质干细胞/分离培养/鉴定/诱导分化Key words
Chicken/UCMSCs/Isolation and in vitro culture/Identification/Differentiation potential分类
生物科学引用本文复制引用
武爽,关伟军,马月辉..鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究[J].华北农学报,2012,27(4):123-129,7.基金项目
转基因生物新品种培育科技重大专项(2011ZX08009-003-006 （）
2011ZX08012-002-06) （）
基本科研业务项目(2011cj-9) （2011cj-9）
国家家养动物种质资源平台项目(2012) （2012）
