华北农学报2012，Vol.27Issue(4)：144-148,5.
抗水稻黑条矮缩病RNA干扰载体的构建及遗传转化
Construction of RNA Interference Vectors for Rice Black-streaked Dwarf Virus and Genetic Transformation
摘要
Abstract
Rice black streaked dwarf disease caused by rice black streaked dwarf virus, lead to dwarf, strong green color of rice,which could seriously influence the yield of rice and food safety. In order to solve the problem, the objective of this study is to use RNA inference (RNAi) strategy to improve the rice resistance ability to virus. Here 5 pairs of black streaked dwarf virus primers were designed according to the published virus sequences in database and related literature, and 5 interference fragment were obtained by RT-PCR, these interference fragments were then built into the modified pUC19 RNA interference vector,and the whole RNA interference fragments were further integrated into the modified binary vector pCAMBIA 1300,which is using ubiquitin as promoter and herbicide resistance Bar gene as a selectable marker. Enzyme digestion and PCR analysis showed that the recombined plasmid were successfully constructed and the transgenic rice seedlings were obtained by agrobacterium-mediated transformation. Our research will shed some light for further investigation of quality and resistance ability improvement of rice to rice black streaked dwarf disease resistance breeding.关键词
水稻黑条矮缩病/RNA干扰载体/Ubi启动子/bar基因/抗病水稻新品种培育Key words
Rice black-streaked dwarf virus/ RNA interference vector/ Ubiquitin promoter/ bar gene/ Cultivation of new germplasm of rice disease resistance分类
生物科学引用本文复制引用
李臻,张贵林,王庆国,柳絮,李海青,姚方印,刘炜..抗水稻黑条矮缩病RNA干扰载体的构建及遗传转化[J].华北农学报,2012,27(4):144-148,5.基金项目
国家转基因植物新品种培育重大专项(2009ZX08001-010B) （2009ZX08001-010B）
