华北农学报2012，Vol.27Issue(4)：168-170,3.
枸杞蚜虫乙酰胆碱酯酶(AChE)底物专一性和有机磷杀虫剂对其的抑制作用
Substrate Specificity of Acetylcholinesterase and the Inhibition of Organophosphate Insecticides to AChE in Aphis sp.
摘要
Abstract
The toxicities of organophosphate( OP) insecticides to Aphis sp. were researched in this paper. It was studied that substrate specificity of acetylcholinesterase and the inhibition of organophosphate insecticides to Aphis sp. . The results showed that the activity of AChE from Aphis sp. hydrolyzing ATCh was the highest, while the affinity of AChE to BuTCh was the closest among the four substrates. The hydrolyzing tendencies of AChE horn- Aphis sp. hydrolyzing four substrates were similar. It was indicated that 3 OP insecticides tested could inhibitor the activity of AChE in Aphis sp. ,and the sensitivity of AChE to omethoate was the highest among the three tested insecticides, and its I50 value was 6. 9 x 10" mol/L. The values of I50 of phoxin and triazophos to AChE were 9. 8 x 10" and 11. 8 x 10 mol/L. The inhibitory curses of three insecticides to AChE were same,and their inhibition abilities increased with time.关键词
枸杞蚜虫/乙酰胆碱酯酶/底物专一性/有机磷杀虫剂/抑制Key words
Aphis sp. /Acetylcholinesterase/Substrate specificity/Organophosphate insecticides/Inhibition分类
农业科技引用本文复制引用
张海珠,李海平,张海燕,常静..枸杞蚜虫乙酰胆碱酯酶(AChE)底物专一性和有机磷杀虫剂对其的抑制作用[J].华北农学报,2012,27(4):168-170,3.基金项目
内蒙古高等学校科学技术研究项目(NJ10056):内蒙古自然基金项目(2010MS0310) （NJ10056）
