华东师范大学学报（自然科学版）Issue(4)：50-60,11.DOI:10.3969/j.issn.1000-5641.2012.04.007
适用于不同尺寸血管的脱细胞方法研究
A general decellularization method for preparing vascular scaffolds from blood vessels of different types, diameters and wall-thicknesses
摘要
Abstract
This study was to build a general decellularization method by combining trypsinization with repeated frozen/thawing treatment for different types of vessels including saphenous vein,carotid artery and aorta.Saphenous vein,carotid artery and aorta were decellularized by trypsinization and repeated frozen/thawing treatment.The efficiencies of cell removal and extracellular matrix (ECM) integrity were examined by Hematoxylin and eosin staining,Masson Trichrome staining and Weigert's staining.A quantitative means based on image analysis software was used to quantify the ECM preservation.Scanning electron microscopy was used to show the extracellular matrix integrity.Histology staining and quantitative analysis demonstrated trypsinization with repeated frozen/thawing treatment deceilularized the saphenous vein,carotid artery and aorta completely. The ECM structure was optimally preserved and integT ity.Scanning electron microscopy examination also showed the ECM was well-preserved and the fibers were dense and orderly.The present results revealed that the trypsinization with repeated frozen/thawing method is a promising one for preparing decellularized vascular scaffolds of different types,sizes and wall-thicknesses of blood vessels.关键词
脱细胞/细胞外基质/隐静脉/颈动脉/主动脉Key words
decellularization/ extracellular matrix/ saphenous vein/ carotid artery/aorta分类
生物科学引用本文复制引用
张红霞,翟万银,张红锋..适用于不同尺寸血管的脱细胞方法研究[J].华东师范大学学报（自然科学版）,2012,(4):50-60,11.基金项目
国家自然科学基金(31070870)This work was supported by grant from the Natural Science Foundation(Grant No.31070870). （31070870）
