郑州大学学报（医学版）2012，Vol.47Issue(5)：616-619,4.DOI:10.3969/j.issn.1671-6825.2012.05.008
山奈酚对CNE-2细胞周期及CyclinB1、Cdk1mRNA表达的影响
Effects of kaempferol on cell cycle status and CyclinB1,Cdk1 mRNA expressions in CNE-2 cells
摘要
Abstract
Aim: To study the effects of kaempferol on cell cycle status and CyclinB1, Cdk1 mRNA expressions in CNE-2 cells. Methods:CNE-2 cells were treated with 0,20,40,60,80,and 100 μmol/L kaempferol. 24,48 and 72 h later, proliferation was determined by MTT assay;24 and 48 h later,cell cycle was detected by flow cytometry;24 h later,the expressions of CyclinBl and Cdkl mRNA were detected by RT-PCR. Results:The CNE-2 cell growth ability was inhibited by kaempferol in a time- and dose-dependent manned Fdose =385. 194,Ftime =237. 324,Finteraetion =13.757,P <0.001 );CNE-2 cells was blocked in G2/M phase ( P<0.05 );the expressions of CyclinB1 and Cdk1 mRNA decreased with the increase of kaempferol dose ( F = 95. 682,154. 871 ,P < 0. 001 ). Conclusion: Kaempferol can block CNE-2 cells in G2/M phase through decreasing the expressions of CyclinBl and Cdkl mRNA,and inhibit the cell proliferation.关键词
山奈酚/CNE-2细胞/细胞周期/Cdk1/CyclinB1/鼻咽癌Key words
kaempferol/ CNE-2 cell/cell cycle/Cdk1/CyclinB1/nasopharyngeal carcinoma分类
医药卫生引用本文复制引用
陈育华,吴国才,王珍,周碧云..山奈酚对CNE-2细胞周期及CyclinB1、Cdk1mRNA表达的影响[J].郑州大学学报（医学版）,2012,47(5):616-619,4.基金项目
湛江市科技攻关计划基金资助项目 2011C3102011 （）
广东医学院青年基金资助项目 XQ1007 （）
