郑州大学学报（医学版）2012，Vol.47Issue(5)：670-674,5.DOI:10.3969/j.issn.1671-6825.2012.05.024
转化生长因子β2对人晶状体上皮细胞间质转分化的影响
Effects of TGF-β2 on epithelial mesenchymal transition in human lens epithelial cells
摘要
Abstract
Aim:To investigate the effects of transforming growth factor-β2( TGF-β2 ) on proliferation and epithelial mesenchymal transition( EMT ) in human lens epithelial cells( HLECs ). Methods: HLECs were cultured and passaged. The HLECs were treated with different concentrations of TGF-β2 for indicated times. The cell survival rate was assayed by MTT. The expressions of α-SMA, E-cadherin and F-actin in the cells treated by 100 ng/L TGF-β2 were analyzed with immunohistochemistry. The expressions of α-SMA and E-cadherin mRNA were detected by RT-PCR. Results: The expressions of E-cadherin after treatment of TGF-β2 was ( 10. 674 ± 3. 233 ), which in control group was ( 23. 352 ± 4. 192 ) ( t = 4. 232,P =0. 013). The expressions of α-SMA and F-actin after treatment of TGF-β2 were ( 17. 613 ±4. 272 )and ( 15.857 ±4. 121 ),respectively, which in control group were 0 and ( 4. 272 ± 1. 538 )( Z = 12. 537, P < 0. 001; t = 7. 186 ,P = 0. 004 ). RT-PCR showed that the expression of E-cadherin mRNA in TGF-β2 group was ( 0. 321 ± 0. 081 ), which in control group was ( 0. 983 ±0.248 )( t = 3. 491 ,P =0. 008 ). The expression of α-SMA mRNA in TGF-β2 group was (0.632 ± 0.158 ), which in control group was ( 0. 097 ± 0.028 )(t = 5. 365,P = 0. 005 ). Conclusion: TGF-β2 could suppress the proliferation and induce EMT of HLECs. EMT of HLECs may be involved in the pathogenesis of posterior capsular opacification.关键词
转化生长因子β2/晶状体上皮细胞/间质转分化/E-钙黏素/α平滑肌肌动蛋白/F-肌动蛋白Key words
TGF-β2/lens epithelial cell/epithelial mesenchymal transition/E-cadherin/α-SMA/F-actin分类
医药卫生引用本文复制引用
朱艳,朱玉广,钟莹莹,杜孝楠,张荣,孙学泉..转化生长因子β2对人晶状体上皮细胞间质转分化的影响[J].郑州大学学报（医学版）,2012,47(5):670-674,5.基金项目
山东省中青年科学家科研奖励基金资助项目 BS2009SW016 （）
山东省卫生厅科技计划基金资助项目 2009HZ105 （）
山东省自然科学基金资助项目Y2008C127 （）
山东省教育厅科技计划基金资助项目 J08LG13 （）
