何文强 1尹继云 1孟晓 1王梅叶 1张文涛 1曲宪东 1李南南1
作者信息
- 1. 武警河南总队医院泌尿外科,郑州,450052
- 折叠
摘要
Abstract
Aim:To investigate the effect of silencing Pim-3 with small interfering RNA( siRNA )on cell proliferation and cell cycle of bladder carcinoma T24 cells. Methods :T24 cells were divided into 3 groups: untreated group, control siR-NA group, and Pim-3 siRNA group. Pim-3 siRNA and control siRNA were utilized to transfect bladder carcinoma T24 cells by Lipofectamine?2000. Western blot was used to detect the protein expressions of Pim-3, Cyclin D1, Cdk2, and P21. CCK-8 kit and flow cytometry were used to examine cell proliferation and cell cycle, respectively. ReSUltS:The protein expressions of Pim-3 ,Cyclin Dl ,Cdk2,and P21 among the 3 groups had significant differences( F = 32. 506,49. 573 ,23. 079 , 44.758,P <0. 05 ). The expressions of Pim-3 ,Cyclin D1, and Cdk2 decreased and P21 increased in Pim-3 siRNA group (P<0. 05 ).The proliferation rate of T24 cells was obviously inhibited at 48,72,96 h after transfection with Pim-3 siRNA ( F = 50.067,132.504,277.389,P<0.001 ).The percentage of G0/G1 ,S,and G2/M phases cells among the 3 groups had significant differences( F = 107. 970,20. 039,66. 872,P <0. 05 ). The percentage of G0/G1 phase of T24 cells in Pim-3 siRNA group was higher than those in untreated group and control siRNA group, whereas S and G2/M phases appeared the opposite result. Conclusion:Pim-3 may be a useful molecular target for therapy of bladder carcinoma.关键词
Pim-3/膀胱癌/T24细胞/小干扰RNA/细胞增殖/细胞周期Key words
Pim-3 /bladder carcinoma/T24 cell/small interfering RNA/cell proliferation/cell cycle分类
医药卫生
