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基于PEG/DEX双水相体系的餐厨垃圾乳酸发酵

叶志隆 蔡伟民 芦敏

环境工程学报2012,Vol.6Issue(9):3299-3304,6.
环境工程学报2012,Vol.6Issue(9):3299-3304,6.

基于PEG/DEX双水相体系的餐厨垃圾乳酸发酵

Lactic acid extraction from kitchen wastes fermentation using aqueous two-phase systems

叶志隆 1蔡伟民 2芦敏3

作者信息

  • 1. 中国科学院城市环境研究所城市环境与健康重点实验室,厦门361021 上海交通大学环境科学与工程学院,上海200240
  • 2. 上海交通大学环境科学与工程学院,上海200240
  • 3. 厦门大学环境与生态学院,厦门361005
  • 折叠

摘要

Abstract

Polyethylene glycol(PEG)/dextran(DEX) aqueous two-phase systems were used to extract lactic acid from kitchen wastes fermentation.Results showed that PEG/DEX system had insignificant effect on the growth of lactic acid bacteria.The variation of PEG and DEX concentrations influenced cells growth and lactic acid biosynthesis indistinctively.Volume ratio kept stable throughout the processes,despite the changes of raw material and product concentrations.Different from little influence of PEG molecular weight on fermentation,the increase of DEX molecular weight from 20 000 to 40 000 led to declines of lactate conversion rate from 0.631 g/(L·h) to 0.518 g/(L·h) and lactic acid concentration from 33 g/L To 22 g/L.A repeated extractive fermentation was carried out with four top-phase replacements.Results presented that when cell density reached the stationary phase in the first extractive fermentation,the lactate yield was maintained above 0.30 g/g per batch and 0.45 g/g in the total process.

关键词

双水相/餐厨垃圾/乳酸/发酵

Key words

aqueous two-phase system/kitchen waste/lactic acid/fermentation

分类

生物科学

引用本文复制引用

叶志隆,蔡伟民,芦敏..基于PEG/DEX双水相体系的餐厨垃圾乳酸发酵[J].环境工程学报,2012,6(9):3299-3304,6.

基金项目

福建省自然科学基金资助项目 ()

福建省科技计划项目 ()

厦门市科技计划项目 ()

环境工程学报

OA北大核心CSCDCSTPCD

1673-9108

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