环境工程学报2012，Vol.6Issue(10)：3747-3751,5.
填埋场中亚硝酸还原酶测定条件的优化
Optimization for determination condition of nitrite reductase in landfill
摘要
Abstract
Nitrogen compounds in the landfill are removed though a series of biological denitrification,in which the nitrite reductase plays a crucial role.Based on the traditional enzymological methods used for the determination of soil nitrite reductase,the conditions for determination of nitrite reductase were optimized with waste samples of landfill.The results showed that the optimum determination conditions for nitrite reductase in landfill were as follows: 25℃ air-dried sample,2.0 mL of 1% NaNO2 and 2.5 mL of 1% glucose,5 min of evacuation time,24 h incubation in 25℃ of culture temperature.The relatively standard deviation(RSD) was less than 1.27% when nitrite reductase was measured under optimization conditions,which indicated higher precision and sensitivity.关键词
填埋垃圾/亚硝酸还原酶/反硝化/优化/正交实验Key words
waste of landfill/nitrite reductase/denitrification/optimization/orthogonal test分类
资源环境引用本文复制引用
樊超,邱忠平,龚正君,海维燕,唐建,华建军..填埋场中亚硝酸还原酶测定条件的优化[J].环境工程学报,2012,6(10):3747-3751,5.基金项目
四川省科技支撑计划 （）
中央高校基本科研业务费专项资金科技创新项目 （）
