环境工程学报2012，Vol.6Issue(11)：3882-3888,7.
高效液相色谱法检测水中5种微囊藻毒素
Detection of 5 variants microcystins in waters using high performance liquid chromatography
摘要
Abstract
A method using high performance liquid chromatography(HPLC) was described for the determination of trace amount of microcystins(MCs),MC-RR,MC-YR,MC-LR,MC-LA and MC-LY) in natural waters.The responses of MCs using two different mobile phases,methanol/trifluoroacetic acid aqueous and methanol/phosphate,were compared.The result showed that the base line was more stable using trifluoroethanoic acid as the buffer solution than phosphate.Furthermore,different ratios of trifluoroethanoic acid were used as water phase,the results indicated that best separation was obtained using 0.02% trifluoroethanoic acid as mobile phase.In addition,five kinds of MCs were separated in 12 min under the condition of gradient mobile phase(methanol/water with 0.02%).The limits of detection(LOD) of this method were 0.015,0.035,0.018,0.032,0.026 μg/L and the limits of quantification(LOQs) were 0.049,0.117 0.062,0.106,0.086 μg/L for MC-RR,MC-YR,MC-LR MC-LA,MC-LY,respectively.The correlation coefficients were ≥0.99 between 0.10~1.50 μg/mL,recoveries of the five variants were in the range of 73.9%~103.3%.关键词
微囊藻毒素-RR/YR/LR/LA/LY/高效液相色谱/天然水体Key words
microcystin-RR/YR/LR/LA/LY/high performance liquid chromatography(HPLC)/natural water bodies分类
资源环境引用本文复制引用
毛敬英,杨敏,狄一安,曹金玲,任立军,许其功,席北斗..高效液相色谱法检测水中5种微囊藻毒素[J].环境工程学报,2012,6(11):3882-3888,7.基金项目
国家“973”重点基础研究发展规划项目 （）
国家“水体污染控制与治理”科技重大专项 （）
