哈尔滨商业大学学报（自然科学版）2012，Vol.28Issue(4)：428-431,438,5.
蛹虫草多糖分离纯化及性质的研究
Study of separation, purification and property of Cordyceps militaris polysaccharides
摘要
Abstract
This paper studied on separation purity and property of the raw Cordyceps militaris polysaccharides ( CMP) , result as follows: the filtrate was concentrated, and used 3 times volume ethanol to precipitate; getting rid of protein at PI with sevag, taking of the color with 10% H2O2; and the raw polysaccharides were separated by DEAE - DE52 chromatography, elution with 0 ～ 0. 2 mol/L NaCl , got the acid polysaccharides which were not separated completely. The polysaccharides were further purified by Sephadex - G200 chromatography, being eluted with distilled water. The polysaccharides were homogenous, which was proved by Sepharose CL -6B. The component of CMP was determined to be composed of D - glucose and D - galactose by paper chromatography. The average molecular weight of CMP was measure by high - pressure liquid chromatography ( HPLC) to be 3. 76×10 ku. The research of bioactivities for CMP was carried out with Kunming strain mice. Polysaccharide on S180 sarcoma inhibition rate was 71. 92%.关键词
蛹虫草/多糖/分离纯化Key words
Cordyceps militaris/ polysccharides/ separation and purity分类
生物科学引用本文复制引用
刘华晶,张旭,史景俊,许修宏..蛹虫草多糖分离纯化及性质的研究[J].哈尔滨商业大学学报（自然科学版）,2012,28(4):428-431,438,5.基金项目
科技部十一五科技支撑项目(2008BADA1B01) （2008BADA1B01）
东北农业大学创新团队发展计划资助(CXT003-2-1) （CXT003-2-1）
东北农业大学大学生科技创新基金. （）
