核农学报2012，Vol.26Issue(5)：746-749,4.
葡萄成熟种子辐射生物学效应研究初报
PRELIMINARY STUDY OF IRRADIATION ON BIOLOGICAL EFFECTS OF GRAPE MATURE SEED
摘要
Abstract
The seeds of two varieties of grapes,Yongyou No.1 and Jufeng,were treated by 60Co γ-rays irradiation of 10,20,50 and 100Gy,respectively.The seedlings from treated seeds were used to detect the germination rate,reduced glutathione,superoxide dismutase,peroxidase,catalyse and seedling growth to investigate the effect of irradiation treatment on the growth and anti-oxidant enzymes of grape and develop the suitable dosage for grape seeds irradiation in mutation breeding of grape improvement.The results showed that the germination rate of augrape seeds were significantly decreased with LD50 of 33Gy.Leaf anti-oxidant enzyme activity assays showed that these four enzyme activities were firstly increased and then significantly decreased with the increase of irradiation dosage with both grape varieties,which may because that low-dosage irradiation can stimulate the antioxidant enzyme activities of seedling leaves of grape,while high dosage irradiation can inhibit these activities.In addition,the stem length of seedlings increased,with the dose(200Gy) increasing.Considering all above results,20~50Gy was recommended as a suitable irradiation dosage of grape seeds.关键词
葡萄/辐照处理/发芽率/抗氧化酶Key words
grape/irradiation treatment/germination rate/anti-oxidant enzymes分类
农业科技引用本文复制引用
王忠华,俞超,陶灵智,钱益芬..葡萄成熟种子辐射生物学效应研究初报[J].核农学报,2012,26(5):746-749,4.基金项目
宁波市重大科技计划项目 （）
宁波市创新团队项目 （）
浙江万里学院重中之重学科开放基金 （）
