核农学报2012，Vol.26Issue(6)：873-878,6.
蓖麻细胞色素P450基因片段的克隆及RNAi表达载体的构建
CLONING OF CYTOCHROME PARTIAL P450 GENE FROM CASTOR(Ricinus Communis L.) AND CONSTRUCTION OF RNAi VECTOR
摘要
Abstract
Sense and anti-sense fragments of castor cytochrome P450 were cloned by PCR.A 525bp sense fragment and a 421bp anti-sense fragment were obtained,which had a homology of 98.85% and 99.51% with the original sequence,respectively.An intermediate vector pHAN-P450S-P450A with hairpin structure was constructed,which was subsequently digested with restriction enzyme,and was ligated into the pBI-121 vector,producing the final plant expression vector.This result provided a foundation for further research of P450 gene expression by RNA interference technology.关键词
蓖麻/P450/RNAi/载体构建Key words
castor/cytochrome P450/RNAi/construction of vector分类
生物科学引用本文复制引用
刘鹏,张立军,张春兰,栾世慧,黄凤兰,陈永胜..蓖麻细胞色素P450基因片段的克隆及RNAi表达载体的构建[J].核农学报,2012,26(6):873-878,6.基金项目
国家自然科学基金项目 （）
内蒙古自然科学基金项目 （）
内蒙古自治区高等学校科学研究项目 （）
