湖南中医药大学学报2012，Vol.32Issue(9)：21-24,4.DOI:10.3969/j.issn.1674-070X.2012.09.005.021.04
秦香止泻方对HRV腹泻乳鼠小肠黏膜一氧化氮浓度的影响
Effects of Qinxiang Zhixie Fang on the concentration of NO in neonatal mice intestinal mucosa with human rotavirus resulted diarrhea
摘要
Abstract
Objective To study the effects and possible mechanisms of Qinxiang Zhixie Fang on nitric oxide concentration in intestinal mucosa of diarrhea neonatal mice caused by human ro-tavirus (HRV). Methods 192 neonatal mice at the age of 3 ~5d were randomly divided into 6 groups as follows: blank group,modeling group,Ribavirin group,Qinxiang Zhixie decoction high-dose group,median-dose group and low-dose group,32 mice in each group,Except for mice in the control group gavaged with culture medium,other mice were inoculated intra-gastrically with an 50 μL(3×l08 PFU) of Wa HRV fluid. After inoculated the virus for 12h,QinXi-ang Zhixie Fang group were gavaged with 50μL different concentrations of QinXiang Zhixie liquid,the Virazole group received the same volume of ribavirin,the control group and modeling group were given the same volume of distilled water and lasted for 8 days HRV antigen in dung was detected by colloidal gold strip,diarrhea symptoms and scores were recorded,The NO concentrations in intestinal mucosa were determined using micro-plate method,The correlation between the diarrhea score and the NO concentration was analyzed. Results The second days after the virus inoculated,the inoculated mice appeared with varying degrees diarrhea. Compared with the modeling group,the duration of diarrhoea were shortened and the degree of diarrhea were relieved in the Virazole group and the Qinxiang Zhixie groups,and the effects presented some dose-effect relationship (all P<0.05). Compared with the control group,the NO concentration was increased significantly in the modeling group (P <0.01). Compared with the modeling group,the NO concentrations were significantly decreased from 1st to 6th days in Qinxiang Zhixie high-dose and median-dose group (all P<0.01). Compared with the Ribavirin group,the NO concentrations were significantly decreased from 1st to 5th days in Qinxiang Zhixie high-dose and median-dose group (all P<0.01). The diarrhea score had positive correlation with the NO concentration (r=0.8012). Conclusion The Qinxiang Zhixie Fang can significantly shorten the duration of diarrhoea caused by HRV infection in neonatal mice,relieve diarrhea extent. The role of the Qinxiang Zhixie Fang may be played by affecting the NO concentration in intestinal mucosa.关键词
秦香止泻方/人轮状病毒/腹泻/一氧化氮/乳鼠/秦皮/木香Key words
QinXiang Zhixie Fang/HRV/Diarrhea/NO/Neonatal mice/Cortex Fraxini/Radix Aucklandiae分类
医药卫生引用本文复制引用
肖瑞飞,谢朝晖,彭芝配,滕久祥,肖璐琼..秦香止泻方对HRV腹泻乳鼠小肠黏膜一氧化氮浓度的影响[J].湖南中医药大学学报,2012,32(9):21-24,4.基金项目
湖南省研究生创新基金项目(CX2011B345) （CX2011B345）
长沙市科技局项目(K100099002-31) （K100099002-31）
湖南省科技厅项目(2011FJ3025) （2011FJ3025）
湖南省教育厅项目(10W021). （10W021）
