湖泊科学2012，Vol.24Issue(5)：712-716,5.
太湖浮游植物细胞裂解速率的酯酶活性法初步研究
Assessing phytoplankton cell lysis rate in Lake Taihu by esterase assay
摘要
Abstract
Phytoplankton lysis rate in three different eutrophic regions of Lake Taihu was measured by the activities of particle and dissolved esterase, as well as the decay rate of the latter, from August 2009 to October 2010. The present results showed that the partieulate, dissolved esterase activity, half life time of dissolved esterase, and cell lysis rates ranged from 0. 58 -35. 15 nmol FDA/( L · h) , 0. 55 -7. 59 nmol FDA/( L · h) , 7 - 75 h, and 0. 02 -0. 77 d -1 , respectively. No significant differences were found in the cell lysis rates among the three sampling sites. The significant linear relationship between Chi.a concentration and partieulate esterase activities suggests that it is feasible to assess phytoplankton cell lysis rate in Lake Taihu by the esterase assay. Furthermore, Chi.a concentration showed the same pattern with water temperature, a significant difference was observed in Chl.a concentration between Meiliang Bay and lake centre. The strong, negative correlation between Chl.a concentration and lysis rate in Gonghu Bay suggests that phytoplankton biomass was affected by the lysis rate in Lake Taihu.关键词
酯酶活性/太湖/细胞裂解速率/浮游植物Key words
Esterase assay/ Lake Taihu/ cell lysis rate/ phytoplankton引用本文复制引用
叶琳琳,史小丽,吴晓东,于洋,张民,孔繁翔..太湖浮游植物细胞裂解速率的酯酶活性法初步研究[J].湖泊科学,2012,24(5):712-716,5.基金项目
国家重点基础研究发展计划“973”项目(2008CB418005,2008CB418000)和国家自然科学基金项目(41001032,40901268)联合资助. （2008CB418005,2008CB418000）
