华西口腔医学杂志2012，Vol.30Issue(5)：463-467,473,6.DOI:10.3969/j.issn.1000-1182.2012.05.004
张、压应力刺激下人牙周膜成纤维细胞早期增殖活性和差异表达基因的变化
Early proliferation changes and differences of gene expression in human periodontal ligament fibroblasts subjected to tensile and compressive stress
摘要
Abstract
Objective The study was aimed to provide insights into cell proliferation ability alteration of human periodontal ligament fibroblasts (HPDLF) under two different types of stimulation, cyclic tensile and compressive stress, and screen the differentially expressed genes to have a comprehensive understanding of the molecular mechanism of changes in proliferation. Methods Primary HPDLF were subjected to cyclic tensile and compressive stress (0.5 Hz, 4 000 μ-strain) for 2 h through a four-point bending strain system. After that, cell proliferation ability was examined by flow cytometry. Then the gene expression profile was investigated by microarray analysis. Results Mechanical stress slowed a down proliferation of cells. S phase cells and proliferation index (PD decreased. Differentially expressed genes were mainly located at nucleus and focus in transcription factor activity related genes, and most of them were involved in the regulation of transcription factors. Among them, more were in group of compressive stress. Conclusion 1) Under mechanical loading, HPDLF proliferation were slowed and cell cycles were arrested. 2) Cell cycle arrest and the slow-down proliferation, which can be viewed as an adaptation and protection mechanism of cells to have more time to decide how to respond to mechanical stimuli, and the differential gene expression reflect the behavior of cells. The re- sult of the response is gene transcription changes at first, and the most important biological response is the nu-clear transcription. 3) HPDLF are more sensitive to cyc-lic compressive stress.关键词
人牙周膜成纤维细胞/张应力/压应力/增殖/基因芯片/差异表达基因Key words
human periodontal ligament fibroblasts/tensile stress/compressive stress/proliferation/microarray/differentially expressed genes分类
医药卫生引用本文复制引用
范晓枫,王羽,李宇,赵志河..张、压应力刺激下人牙周膜成纤维细胞早期增殖活性和差异表达基因的变化[J].华西口腔医学杂志,2012,30(5):463-467,473,6.基金项目
国家自然科学基金资助项目(10772128) （10772128）
高等学校博士学科点专项科研基金资助项目(20060610083) （20060610083）
