四川大学学报（医学版）2012，Vol.43Issue(5)：651-656,6.
狭叶重楼甾体皂苷类化学成分PSⅡ体外抗肿瘤作用的观察
Observation on Anticancer Function of PS Ⅱ Isolated from Rhizoma Paridis
摘要
Abstract
Objective To isolate compound Paris saponin II (PS II ) from Rhizoma Paridis and observe its antitumor activity. Methods PS II was isolated and determined by electrospray ionization-mass spectrometry,1 H and 13C nuclear magnetic resonance spectral analysis. PS II was used to treat cancer cells to analyze the toxicity and relative time-and dose-dependent manner. Apoptosis of cells were investigated by flow cytometry and TUNEL assay. Western blotting was used to analyze the effects of PS H to MAPKs and mitochondrial apoptotic pathway. Results The anti-tumor activities of PS II on ovarian carcinoma cell line SKOV3 were investigated. The IC50 of PS II to SKOV3 was 4. 81umol/L. Increased apoptosis rate in a dose- and time-dependent manner was observed by Flow cytometry and TUNEL. The activity of ERK1/2 was inhibited by PSII and increased expression of cytochrone c, caspase-3 and caspase-9 was also noticed after the treatment of PSII . Conclusion PSII can inhibit the growth of ovarian cancer cells SKOV3 through affecting the key proteins on MAPKs pathway and inhibiting the activity of ERK1/2 and eventually eliciting programmed cell death of SKOV3. The mitochondrial apoptotic pathway may be involved in the PS II induced apoptosis.关键词
PSⅡ/凋亡/线粒体通路/丝裂原激活蛋白酶通路Key words
Paris saponin II/Apoptosis/Mitochondrial pathway/MAPKs pathway引用本文复制引用
肖雪,杨媚,白鹏,陈新莲,刘珊玲,邹娟,王和..狭叶重楼甾体皂苷类化学成分PSⅡ体外抗肿瘤作用的观察[J].四川大学学报（医学版）,2012,43(5):651-656,6.基金项目
国家自然科学基金(No.81001159)资助 （No.81001159）
