四川大学学报（医学版）2012，Vol.43Issue(5)：779-781,785,4.
正相HPLC-MS/MS测定人血浆中多奈哌齐对映体浓度
Determination of Donepezil Enantiomer in Human Plasma by Normal-HPLC-MS/MS
摘要
Abstract
Objective To develop and validate a normal phase HPLC-MS/MS method for the determination of donepezil enantiomer in human plasma. Methods Donepezil was extracted from plasma by n-hexane ! isopropanol (98:2, V/V) with lidocaine serving as an internal standard. The analytes went through the column of CHIRALCEL OJ-H (250 mmX4. 6 mm, 5 um) with mobile phase n-hexane:n-propanol:diethylamine (60:40: 0. 1 .V/V/V). Donepezil enantiomer was determined by API 3000 in MRM mode. Results The retention time of S-DN and R-DN were 15. 56 min and 18. 41 min, respectively. The calibration curves were linear in a range from 0. 051 to 7. 596 ng/mL for S-DN, and from 0. 049 to 7. 404 ng/mL for R-DN, respectively, both with more than 0. 99 correlation coefficients. The relative recovery were 95. 10%-103. 70% for S-DN and 93. 58%-98. 00% for R-DN, respectively; the pretreatment recovery were 58. 42%-61. 08% for S-DN and 53. 24%-61. 87% for R-DN, respectively;; the within-day RSD ranged from 8. 35% to 11. 28% for S-DN and from 6. 78% to 11. 58% for R-DN, respectively; the between-day RSD ranged from 5. 82% to 9. 02% for S-DN and from 6. 87% to 9. 19% for R-DN, respectively. Conclusion This normal phase HPLC-MS/MS method is simple, rapid, sensitive and accurate for the determination of donepezil enantiomer in human plasma and is suitable for pharmacokinetic studies of donepezil enantiomers.关键词
多奈哌齐/对映体/正相质谱Key words
Donepezil/Enantiomers/Normal HPLC-MS/MS引用本文复制引用
余勤,向瑾,石玲子,梁茂植,秦永平,南峰..正相HPLC-MS/MS测定人血浆中多奈哌齐对映体浓度[J].四川大学学报（医学版）,2012,43(5):779-781,785,4.基金项目
四川省科技支撑计划项目(No.2010SZ0271)资助 （No.2010SZ0271）
