渔业科学进展2012，Vol.33Issue(4)：57-63,7.
养殖大菱鲆黑瘦症的病原菌鉴定及杀菌中草药筛选
Causative pathogen of dark-thin syndrome in cultured Scophthalmus maximus and Chinese herbal medicine screening for the bactericidal effect
摘要
Abstract
Dark-thin syndrome often occurred at stages of larval turbot Scophthalmus maximus, causing lower metamorphosis and more than 80% of mortality. One bacterial strain was isolated from the diseased larvae, and it was confirmed to be the pathogen causing the disease through artificial infection tests. The pathogenic bacterium was identified as Vibrio splen-didus by conventional physiological and biochemical tests as well as 16S rDNA sequence analysis. In addition, histopathological studies demonstrated that the bacterium could cause infectious changes in the liver, kidney, spleen, intestine and brain. The effective components of 11 Chinese herbs were extracted respectively by the methond of decoction, and they were tested for the susceptibility against V. splendidus. The results showed that Galla chinensis and Radix pulsatillae had the best inhibitory effect on the bacterium, with minimal bactericidal concentration (MBC) of 25mg/ml. MBC for both Phellodendronchinen.se and Herba andrographitis was 50mg/ml. While Chinese angelica , Radix codonopsisand , Eucommia bark , Codex fraxini , Flos Inicerae , Bosxvellia carterii and Commiphora molmol had the weakest inhibition effect with MBC more than l00mg/ml. The preliminary tests indicated that G. chinensis and R. pulsatillae were good treatments by oral and immersion administration.关键词
大菱鲆/黑瘦症/病原菌/中草药/最小抑菌浓度(MIC)/最小杀菌浓度(MBC)Key words
Turbot Scophthalmus maximus/ Dark-thin syndrome/Pathogen Chinese herbs/Minimal inhibitory concentration(MIC) Minimal bactericidal concentration (MBC)分类
农业科技引用本文复制引用
王印庚,任海,张正,王岚,荣小军,廖梅杰,陈霞..养殖大菱鲆黑瘦症的病原菌鉴定及杀菌中草药筛选[J].渔业科学进展,2012,33(4):57-63,7.基金项目
国家科技支撑计划项目(2012BAD07B03)资助 （2012BAD07B03）
