解放军医学杂志2012，Vol.37Issue(10)：958-961,4.
N-乙酰-L-半胱氨酸对多器官功能障碍综合征模型小鼠肺树突细胞损伤的保护作用
Protective effect of N-acetylcysteine on lung dendritic cells against damage in multiple organ dysfunction syndrome model
摘要
Abstract
Objective To explore the protective effect of N-acetylcysteine (NAC) on lung dendritic cells (DCs) from damage in multiple organ dysfunction syndrome (MODS) in mouse model. Methods Animal model of MODS was reproduced by injecting zymosan into the peritoneal cavity of BALB/c mice, and the mice were thereafter randomly divided into zymosan group, zymosan + NAC group and control group, 20 for each group. Myeloperoxidase MPO activity in lung tissue was measured by biochemical analysis 48 hours after modeling. Pathological changes of the lung were observed under light microscope. Lung DCs were isolated by density gradient centrifugation and CDllc+ immunomagnetic beads. DCs' phenotypes of MHC- H / I Ad and CD86 were analyzed by flow cytometry. Apoptosis of DCs was detected by flow cytometry with double labeling of Annexin V and 7-AAD. Results Compared with control group, the MPO activity in lung tissue remarkably increased in zymosan group (P<0.05). Infiltration with a large number of neutrophilic granulocytes was found in lungs, suggesting serious inflammation and injuries in lung. The expressions of MHC- II / I -A and CD86 on DC surface and the percentage of DC apoptosis increased dramatically (P<0.05). Compared with zymosan group, the MPO activity was remarkably lowered and the lung injury was alleviated (P<0.05); the number of infiltrating neutrophilic granulocytes markedly decreased, and the expressions of MHC- II / I -A and CD86 on the DC surface and the percentage of DC apoptosis were mitigated dramatically (P<0.05) in zymosan + NAC group. Conclusion NAC in vivo could inhibit DC activation-induced apoptosis and relieve lung injury, thus it possesses protective effect on lung DCs against damage in MODS model.关键词
N-乙酰-L-半胱氨酸/多器官衰竭/树突细胞/细胞凋亡Key words
N-acetylcysteine/ multiple organ failure/ dendritic cells/ apoptosis分类
医药卫生引用本文复制引用
王宏伟,陆江阳,田光,杨毅,康佳蕊..N-乙酰-L-半胱氨酸对多器官功能障碍综合征模型小鼠肺树突细胞损伤的保护作用[J].解放军医学杂志,2012,37(10):958-961,4.基金项目
国家自然科学基金(81000848) （81000848）
全军医学科研"十一五"规划面上项目(06MB307) （06MB307）
