激光生物学报2012，Vol.21Issue(1)：16-21,6.DOI:10.3969/j.issn.1007-7146.2012.01.004
复合调控Wx与SBE3基因对水稻籽粒直链淀粉含量的影响
Effects of Overexpression of Wx gene and RNA Interference of SBE3 Gene Compound Expression on Amylose Content in Rice
摘要
Abstract
Granule-bound starch synthase( GBSS) and starch branching enzyme 3 ( SBE3 ) are two major enzymes involved in starch biosynthesis in rice endosperm. The granule-bound starch synthase encoded by the Wx gene, and the starch branching enzyme 3 were encoded by the SBE3 gene. Their gene expression level influence the abundance ratio of amylose and amylopectin immediately. In order to study the change of the amylose content that is caused by overexpression of Wx gene and RNAi of SBE3 gene expression in rice, this multiple construct was introduced into the rice variety of Oryza sativa L. by Agrobacterium-medialed transformation. Transgenic plants carrying the multiple construct were obtained and verified by PCR, and the expression of Wx was significantly increased in transgenic immature seeds, while the expression of RBE3 was significantly reduced in transgenic immature seeds. Grain transparency of these transgenic plants was significantly reduced. Analysed the amylase content in endosperm of these transgenic plants, the amylose content was increased. The average increase level was about 45% . Thousand-grain weight of these transgenic plants was not change significantly. Genetic analysis showed that these genetically modified strain most can stable genetic.关键词
水稻/直链淀粉/Wx/SBE3/RNA干涉/过量表达Key words
rice/ amylose/ Wx/ SBE3 / RNA interference/ overexpression分类
生物科学引用本文复制引用
唐秀丽,周玉琼,郑丽莉,江海洋,朱苏文..复合调控Wx与SBE3基因对水稻籽粒直链淀粉含量的影响[J].激光生物学报,2012,21(1):16-21,6.基金项目
国家科技支撑计划(2009BADA6B01-3) （2009BADA6B01-3）
国家自然科学基金(11075001) （11075001）
