激光生物学报2012，Vol.21Issue(3)：233-237,5.DOI:10.3969/j.issn.1007-7146.2012.03.008
秋水仙碱诱导甘草四倍体的研究
Study on Tetraploid Induction of Glycyrrhiza uralensis by Colchicines
王丽艳 1郭永霞 1荆瑞勇 1潘翠翠 1殷奎德1
作者信息
- 1. 黑龙江八一农垦大学,黑龙江大庆 163319
- 折叠
摘要
Abstract
The mature seeds of Glycyrrhiza inflata Batalin were used as material, the seeds treated with budding for 12 h, 24 h, 36 h were immersed in 0.05% , 0.076% and 0. 1% colchicines solutions for 12 h, 24 h, 36 h respectively for inducing tetraploid and the chromosome numbers of the regenerated plantlets were examined. The results showed that the treatment with 0. 075% colchicine solution for 24 h after 24 h of budding would be the best combination and the mutation rate reached 41% . The chromosome number of cell become from 2n =2x = 16 to 2n =4x =32.关键词
胀果甘草/秋水仙碱/诱导/四倍体Key words
Glycynhiza inflata Batalin/ colchicine/ introduction/ tetraploid分类
农业科技引用本文复制引用
王丽艳,郭永霞,荆瑞勇,潘翠翠,殷奎德..秋水仙碱诱导甘草四倍体的研究[J].激光生物学报,2012,21(3):233-237,5.
