激光生物学报2012，Vol.21Issue(3)：283-288,6.DOI:10.3969/j.issn.1007-7146.2012.03.017
云斑尖塘鳢AFLP分析技术的建立
AFLP Molecular Marker Technology System of Oxyeleotris marmorata
摘要
Abstract
AFLP analysis system for Oxyeleotris marmorata was established by optimizing procedures that include adapter ligation reaction, pre-amplification and selective amplifications. The results showed that quite steady results could be achieved by using phosphated-adapter, additional extension procedure before pre-amplified PCR and pre-amplified products diluted 20 times. 14 pairs of selective primer combinations which revealed sufficient amplified bands with high resolution were selected from 48 pairs of primers, clear fingerprints (50-70) were obtained. Those 14 pair primers could be used to study the population genetic diversity and molecular assisted selection of 0. marmorata.关键词
AFLP/云斑尖塘鳢/反应体系Key words
AFLP/ Oxyeleotris marmorata/ reaction system分类
生物科学引用本文复制引用
朱晓平,骆剑,王茜,尹绍武..云斑尖塘鳢AFLP分析技术的建立[J].激光生物学报,2012,21(3):283-288,6.基金项目
海南省自然科学基金项目(310037) （310037）
海南省海南大学科研基金项目(kyqd1018) （kyqd1018）
海南大学”211”工程研究生创新平台课题 （）
