经济动物学报2012，Vol.16Issue(3)：129-132,4.
梅花鹿鹿茸表皮生长因子基因原核表达载体的构建及鉴定
Construction and Identification of Prokaryotic Expression Plasmid for Epidermal Growth Factor Gene of Sika Deer
摘要
Abstract
The clone vector pMD-18T-EGF was constructed successfully by T-A clone technique and was digested with EcoR Ⅰ and HindⅢ, and the prokaryotic expression vector pE332a was also cut, then the gene was cloned into the vector. Finally a recombinant plasmid named pET32a-EGF was obtained, and identified by PCR, restriction enzyme analysis and sequencing.关键词
梅花鹿/鹿茸,表皮生长因子/原核表达载体Key words
sika deer/antler/epidermal growth factor/prokaryotic expression vector分类
生物科学引用本文复制引用
苏凤艳,宗颖,何忠梅..梅花鹿鹿茸表皮生长因子基因原核表达载体的构建及鉴定[J].经济动物学报,2012,16(3):129-132,4.基金项目
吉林省教育厅项目[2009第（66）号] （）
吉林省自然科学基金项目 （）
