经济动物学报2012，Vol.16Issue(3)：154-158,162,6.
东北林蛙体表两种常驻细菌的分离鉴定
Isolation and Identification of Bacteria on the Surface of Rana dybowskii
摘要
Abstract
The objective of this study was to characterize the resident bacterial flora on the skin of healthy Rana dybowskii living in natural field state. After rinsed transient bacteria from the skin with sterile wa- ter, resident bacteria from frog skin were sampled using sterile swabs and cultured, and single colony was isolated and purified. After observation of colony morphology, biochemical reactions and sequencing of the 16S rDNA gene were used to identify the bacteria. Then two bacterial species from Rana dybowskii skin were identified to be related to the species Acinetobacter calcoaceticus and Pseudomonas alcali- genes by biochemistry and molecular methods. Both the bacteria were found gram negative and common in the environment. This results suggested that Rana dybowskii skin resident bacteria came from the envi- ronment and might have a protective role in preventing infection.关键词
东北林蛙/体表/细菌/生化/16S/rDNAKey words
Rana dybowskii/skin/bacteria/biochemistry/16S rDNA分类
生物科学引用本文复制引用
张建平,肖向红,苗慧敏..东北林蛙体表两种常驻细菌的分离鉴定[J].经济动物学报,2012,16(3):154-158,162,6.基金项目
国家自然科学基金项目 （）
黑龙江省自然科学基金重点项目 （）
