经济动物学报2012，Vol.16Issue(3)：168-171,4.
馆藏泽蛙标本壶菌病原实时PCR检测与系统发育分析
Real-time PCR Detection and Phylogenetic Analysis for Batrachochytrium dendrobatidis in Rana limnocharises from Samples of a Museum
摘要
Abstract
In order to research and prove the chytridiomycosis of amphibians in our country in the history, review the origin of the Batrachochytrium dendrobatidis from the aspects of time and systematic evolution, 39 Rana limnocharises which were collected from Guangdong province and held in museum in 1982 were screened by Taqman-MGB fluorescence probe quantitative polymerase chain reaction to detect the pathogens; and the products of QPCR were cloned and sequenced to identify the origin of the pathogens by sequence alignment and phylogenetic analysis. Finally we got the standard curve: Y = - 3.1 X + 32.65 and the related coefficient: R2 = 0.999 8. There were 12 positive samples were gotten to report the detection rate as 30.8 %. Meanwhile the phylogenetic analysis indicated that a certain extent differentia- tion of the Batrachochytrium dendrobatidis in our country existed. One type of the fungi had altitudinal genetic relationship with the strains from the North America, South America and Europe. Another one was obviously different from the strains in the other areas of the world with special characteristics. The research boosted the earliest record of the Batrachochytrium dendrobatidis in China to 1980s.关键词
泽蛙/馆藏标本/壶菌/实时PCR/系统发育分析Key words
Rana limnocharises/museum specimens/Batrachochytrium dendrobatidis/real-time PCR/phylogenetic analysis分类
生物科学引用本文复制引用
曾朝辉,白世卓,朱蕴绮,王晓龙..馆藏泽蛙标本壶菌病原实时PCR检测与系统发育分析[J].经济动物学报,2012,16(3):168-171,4.基金项目
北京市科学技术研究院“萌芽后续”人才培养计划项目 （）
