江西农业大学学报2012，Vol.34Issue(4)：718-723,6.
银杏内生真菌菌株R2的鉴定及其抑菌活性
Identification of Endophytic Fungi Strain R2 from Ginkgo biloba and Its Antimicrobial Activity
摘要
Abstract
The antimicrobial activity of 35 endophytic fungi isolated from traditional Chinese medicinal plant Ginkgo biloba were detected by the method of cylinder plate test with Staphylococcus aureus, Escherichia coli and Bacillus subtilis as indicator microorganisms. Observation on the morphological characters and internal transcribed spacers ribosomal DNA (ITS-rDNA) sequence analysis were conducted to identify the strain. The results showed that the extracts of 8 isolated entophytic fungi strain L1, L4, L5 , L7 , T2 , T3 , Rl and R2 had antibiotic activity against some indicator microorganisms. Especially the extract of strain R2 obviously exhibited strongest antibiotic activity against those indicator microorganisms, the inhibition zones diameters were 20 mm, 20 mm and 21 mm respectively. Based on the morphological characters and ITS-rDNA sequence analysis, the endophytic fungi strain R2 was identified as Cladosporium oxysporum.关键词
银杏/内生真菌/抑菌活性/鉴定Key words
Ginkgo biloba/ endophytic fungi/ antimicrobial activity/ identification分类
农业科技引用本文复制引用
汪涯,李希茜,张志斌,颜日明,曾庆桂,朱笃..银杏内生真菌菌株R2的鉴定及其抑菌活性[J].江西农业大学学报,2012,34(4):718-723,6.基金项目
江西省亚热带植物资源保护与利用重点实验室开放基金资助(YRD201203) （YRD201203）
