江西农业大学学报2012，Vol.34Issue(4)：769-774,6.
不同功能性寡糖组合对锦江黄牛瘤胃固相微生物多样性的影响
Effects of Different Functional Oligosaccharide Combinations on Diversity of Rumen Solid Bacteria in Jinjiang Cattle
摘要
Abstract
This experiment was conducted to study the effects of different functional oligosaccharide combinations on the band numbers of DGGE-DNA fingerprints of rumen solid bacteria in Jinjiang cattle. Three healthy Jinjiang cattle with permanent rumen cannula and weight of ( 300 ± 20) kg were selected. By self-contrasted method, the test was divided into three periods, the first period: the control period, basal diet was fed without any addtional oligosaccharides, the second period, the basal diet added with GT1 of functional oligosaccharide combination was fed, the third period, the basal diet added with GT2 of functional oligosaccharide combination was fed, the experiment lasted 14 days for each period. The band number of PCR-DGGE fingerprints and similarity of rumen solid bacteria in Jinjiang cattle were analyzed. The results showed that compared with the control period, the effects of functional oligosaccharide combinations of GT1, GT2 on the band numbers of DGGE-DNA fingerprints of rumen solid bacteria in Jinjiang cattle were not obvious. The similarity degree of the three trial cattle was higher in the three periods. The effects of different functional oligosaccharide combinations on diversity of rumen liquid bacteria in Jinjiang cattle remain to be determined.关键词
功能性寡糖组合/变性梯度凝胶电泳(DGGE)/锦江黄牛/瘤胃固相微生物/多样性Key words
functional oligosaccharides/DGGE/ Jinjiang cattle/rumen solid bacteria/diversity分类
农业科技引用本文复制引用
闵力,瞿明仁,戈婷婷,钟志勇,刘立恒..不同功能性寡糖组合对锦江黄牛瘤胃固相微生物多样性的影响[J].江西农业大学学报,2012,34(4):769-774,6.基金项目
国家现代肉牛产业技术体系项目(CARS-38)、国家自然科学基金(30960254)和江西省教育厅项目(GJJ10405,2009GJJ10409) （CARS-38）
