江西农业学报2012，Vol.24Issue(8)：47-50,4.
早熟梨新梨7号胚培养直接成苗的影响因素探讨
Study on Factors Affecting Plantlet Regeneration Directly from Cultivated Embryos of Early- mature Pear Variety Xinli No.7
刘艳 1吴翠云 2袁丙岑3
作者信息
- 1. 新疆生产建设兵团农业建设第二师农业科学研究所,新疆库尔勒841005
- 2. 塔里木大学植物科学学院,新疆阿拉尔843300
- 3. 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300
- 折叠
摘要
Abstract
Taking early - mature pear variety Xinli No. 7 as test material, the author studied the influences of basic culture medium, embryo age, hormone and low temperature pre -treatment on the plantlet regeneration directly from the cultivated young embryos in vitro. The results indicated that the embryo germination rate and cotyledon green - turning on MS medium were similar to those on White medium. However, the rooting rate and seedling - forming rate at the latter culture stage on MS medium were better than those on White medium. The optimum hormone concentration and medium combination was MS ＋2. 0 mg/L 6 - BA ＋0.1 mg/L NAA. The culture of young embryos with the age of more than 85 d was more easily successful. The pre - treatment by low temperature had greater promotive effects on embryo germination and seedling - forming of 65 - day - old and 75 - day - old embryos than those of 85 - day - old embryos, and the improving effects of low temperature pre - treatment on the embryo germination and cotyledon green - turning were greater than those on the rooting rate and seedling - forming rate.关键词
新梨7号/胚培养/胚龄/成活率/成苗Key words
Xinli No. 7/ Embryo culture/ Age of embryo/ Germination rate/ Seedling - forming分类
农业科技引用本文复制引用
刘艳,吴翠云,袁丙岑..早熟梨新梨7号胚培养直接成苗的影响因素探讨[J].江西农业学报,2012,24(8):47-50,4.
