昆虫学报2012，Vol.55Issue(7)：860-868,9.
细纹豆芫菁3-羟甲基戊二酰辅酶A-还原酶基因全长cDNA克隆及生物信息学分析
Cloning and bioinformatical analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from the blister beetle Epicauta mannerheimi ( Coleoptera: Meloidae)
摘要
Abstract
3-Hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR) is a key enzyme of the mevalonate pathway. Obtaining HMGR gene information is the basis of studying the relationship between cantharidin biosynthesis and the mevalonate pathway. In this study, a full-length cDNA of the HMGR gene was cloned from the blister beetle Epicauta mannerheimi ( Maklin) by RACE technology, which was named EmHMGR (GeneBank accession no. JQ690539). The full-length of EmHMGR is 3 118 bp with an ORF of 2 526 bp, containing a 5'-untranslated region (5'-UTR) of 178 bp and a 3'UTR of 414 bp. It encodes a deduced protein of 842-amino acid residues, with a predicted molecular mass of 92.8 kDa and isoeletric point (pI) of 6.0. Its predicted molecular formula is C4135H6604N1098O1216S50. Bioinformatical analysis showed that its instability index is 43. 37 and the GRAVY 0.091, suggesting that EmHMGR is an unstable hydrophobic protein. The deduced protein EmHMGR has the conserved functional domain of HMGR_Class I and the sterol-sensing domain, and shares more than 50% amino acid identity with HMGRs from other insects. Phylogenetic analysis showed that EmHMGR has the closest relationship with HMGRs of chrysomelids. This study provides the basis for further research on the biosynthetic pathway of cantharidin in the blister beetles.关键词
细纹豆芫菁/3-羟甲基戊二酰辅酶A-还原酶/RACE/克隆/生物信息学分析/甲羟戊酸途径Key words
Epicauta mannerheimi/ 3-hydroxy-3-methylglutaryI coenzyme A reductase (HMGR)/ RACE/ cloning/ bioinformatical analysis/ mevalonate pathway分类
生物科学引用本文复制引用
姜鸣,霍棠,吕淑敏,张雅林..细纹豆芫菁3-羟甲基戊二酰辅酶A-还原酶基因全长cDNA克隆及生物信息学分析[J].昆虫学报,2012,55(7):860-868,9.基金项目
公益性行业(农业)科研专项(200903052) （农业）
中国博士后科学基金(20090451405) （20090451405）
