昆虫学报2012，Vol.55Issue(8)：981-987,7.
小菜蛾不同地理种群遗传多样性的ISSR标记研究
Genetic diversity of different geographical populations of Plutella xylostella (Lepidoptera: Plutellidae) from China based on ISSR analysis
摘要
Abstract
The genetic diversity of eight characteristic geographical populations of Plutella xylostella from China was investigated with ISSR method using fifteen selective primers in this study in order to clarify the genetic diversity of different geographic populations of P. xylostella. The results showed that total 395 ISSR loci, of which 89. 11 % are polymorphic, were obtained, and the characteristic ISSR pattern for each P. xylostella individual was detected. The proportion of polymorphic bands (P) , Shannon' s index (I) and Nei' s gene diversity index (H) of P. xylostella displayed a high degree of genetic diversity both at the species level (P = 89. 11 % , H = 0. 2706 , I= 0.4286) and at the population level (P = 88. 80% , H= 0.2759, I = 0.4349). The genetic diversity of P. xylostella was higher in areas of southern China ( Yunnan and Hubei) than in areas of northern China ( Beijing, Tianjin, Shandong, Heilongjiang, and Gansu) . The intra-population genetic variation was the highest in Nankou of Beijing, and the lowest in Haikou of Hainan and Lanzhou of Gansu. In additional, 5. 66% genetic variations are from intra-population and 94. 34% from inter-population (Nm =8.3399). No obvious genetic differentiation exists among the eight characteristic geographical populations of P. xylostella in this study. The results provide a valuable basis for the pesticide resistance control and the integrated management of the field population of P. xylostella.关键词
小菜蛾/地理种群/遗传多样性/ISSR/聚类分析Key words
Plutella xylostella/ geographical population/ genetic diversity/ ISSR/ cluster analysis分类
生物科学引用本文复制引用
朱勋,杨家强,吴青君,李建洪,王少丽,郭兆将,刘雅婷,张友军,杨峰山..小菜蛾不同地理种群遗传多样性的ISSR标记研究[J].昆虫学报,2012,55(8):981-987,7.基金项目
“十二五”科技支撑计划课题(2012BAD19B06) （2012BAD19B06）
农业部公益性行业专项(200803001) （200803001）
蔬菜有害生物控制和优质栽培北京市重点实验室资助课题 （）
哈尔滨市科技创新人才研究专项资金优秀学科带头人项目(2007RFXXN048) （2007RFXXN048）
黑龙江教育厅面上项目(11511273) （11511273）
