昆明医学院学报2012，Vol.33Issue(7)：11-14,4.
IRF4逆转录病毒表达载体的构建及鉴定
Construction and Identification of Retroviral Expression Vector Harboring Murine IRF4
摘要
Abstract
Objective To construct a recombinant retroviral vector bearing murine IRF4 cDNA and establish a GP+E86 cell line for stable expression of IRF4. Methods IRF4 cDNA fragment was amplified from a plasmid containing IRF4 ORF by PCR, and cloned into MSCV-IRES-GFP retroviral vector. The recombinant vector was transfected into 293T. The virus from the supernatant was harvested and used to infect GP+E86 cell line. Expression of IRF4 in infected cells was analyzed by fluorescence activated cell sorter and confirmed by Western blotting. Results IRF4 cDNA was successfully cloned into MSCV-IRES-GFP retroviral vector. The resulting retrovirus successfully infected GP+E86 cell line. Conclusion The recombinant retroviral vector bearing murine IRF4 cDNA has been successfully constructed, and the expression of exogenous IRF4 gene in mouse GP+E86 cell line has been achieved.关键词
IRF4/逆转录病毒载体/小鼠Key words
IRF4/Retrovirus/Mice分类
生物科学引用本文复制引用
何颖红,赵文珍,杨勇琴,王一心..IRF4逆转录病毒表达载体的构建及鉴定[J].昆明医学院学报,2012,33(7):11-14,4.基金项目
国家自然科学基金资助项目(81060248) （81060248）
云南省自然科学基金资助项目(2010CD084) （2010CD084）
