临床肝胆病杂志2012，Vol.28Issue(9)：677-680,4.
消黄方对二甲基亚硝胺致肝纤维化大鼠肺组织氧化应激及巨噬细胞激活的影响
Effects of Xiaohuang decoction on anti-oxidative stress and macrophage activation in lungs of rats with DMN -induced liver fibrosis
摘要
Abstract
Objective To observe the effects of Xiaohuang decoction (XHD) on anti - oxidative stress and macrophage activation in lungs of rats with dimethylnitrosamine (DMN) -induced liver fibrosis. Methods Liver fibrosis was induced in male Wistar rats by DMN injection , three days a week for four weeks and divided into two groups: untreated model group and XHD - treated group. XHD or distilled water was administered eight times a day for two weeks. All rats were sacrificed at the end of the 4th week and lung tissues harvested for analysis, Oxidative stress indicators [ superoxide dismutase ( SOD) , glutathione ( GSH) , and malondialdehyde (MDA) ] in the lung were detected by biochemical assays. CD68 expression in the lung was detected by Western blot, immunohistochemistry, and real -time quantitative PCR. Results In the DMN - induced rats, SOD activity and GSH content had decreased remarkably after two weeks and was maintained at a low level state out to four weeks, but MDA content had increased after two weeks. XHD treatment significantly reduced the DMN - enhanced MDA content and notably increased the DMN - inhibited SOD activity and GSH content after four weeks. Immunohistochemical analysis of uninduced normal control rats revealed that GD68 was localized to the alveoli and weakly expressed. However, in DMN lung, the CD68 expression was strong after two weeks. XHD - treated DMN rats had weak CD68 - positive staining in lung. Conclusion Oxidative stress and activation of macrophages occurred in the lung during DMN - induced hepatic fibrosis. XHD treatment inhibits oxidative stress and lung macrophage activation.关键词
肝硬化/消黄方/巨噬细胞,肺泡/二甲基亚硝胺Key words
liver cirrhosis/ Xiaohuang decoction/ macrophages, alveolar/ dimethylnitrosamine分类
医药卫生引用本文复制引用
刘成,杨宗国,陈晓蓉..消黄方对二甲基亚硝胺致肝纤维化大鼠肺组织氧化应激及巨噬细胞激活的影响[J].临床肝胆病杂志,2012,28(9):677-680,4.基金项目
国家十一五科技重大专项子课题(2009ZX10005-019) （2009ZX10005-019）
上海市科学技术委员会项目(No.25382)资助 （No.25382）
上海市科委引导类项目(10411963000) （10411963000）
中央高校基本科研业务费专项资金资助(10F115) （10F115）
