临床肝胆病杂志2012，Vol.28Issue(9)：685-688,4.
软肝化坚颗粒对肝纤维化模型C57小鼠肝组织α-SMA、TGFβ1表达和Ⅰ型胶原水平的影响
Effects of Ruangan Huajian granule on hepatic expression of α-SMA, TGFβ1, and COL-Ⅰ in C57 mice with experimental hepatic fibrosis
摘要
Abstract
Objective To observe the effect of Ruangan Huajian granule on hepatic expression of alpha - smooth muscle actin (α - SMA) , tumor growth faotor - beta 1 (TGF(3l) , and collagen Ⅰ (COL - Ⅰ) using a C57 mouse model of experimental hepatic fibrosis. Methods Forty C57 mice were randomly divided into four groups (n = 10 each) ; uninduced normal control group, model control group, model treatment group with low -dose (0. 1 g/day for six weeks) Ruangan Huajian granule, model treatment group with high -dose (0.2 g/day for six weeks) Ruangan Huajian granule. Hepatic fibrosis was induced by intraperitoneal injection of 10% carbon tetrachloride. The expression of α - SMA, TGFβ1, and COL -Ⅰ in liver tissue of mice was observed by immunohistochemistry. The liver tissues were also scored for fibrosis stage by evaluating morphological changes detected upon hematoxyiin - eosin and Masson trichrome staining. Results There were significantly higher levels of α -SMA, TGFfU , and COL- Ⅰ expression and scores of fibrosis stage in the hepatic fibrosis mice than in the normal control mice (P < 0. 05 ) and in the model control group than in either the low - dose or high - dose model treatment groups (P <0. 05 ) . Therc was no significant difference between the low -dose model treatment group and the high -dose model treatment group (P >0.05), Conclusion Ruangan Huajian granule may alleviate the degree of fibrosis in C57 mice with experimental hepatic fibrosis by reducing hepatic expression of a - SMA and TGFpl. Furthermore, Ruangan Huajian granule may depress hyperplasia by reducing COL -I expression.关键词
软肝化坚颗粒/肝硬化/α-平滑肌肌动蛋白/转化生长因子β/Ⅰ型胶原Key words
Ruangan Huajian granule/ liver cirrhosis/ α -smooth muscle actin/ transforming growth factor beta/ collagens Ⅰ分类
医药卫生引用本文复制引用
叶立红,王翀奎,杨莉,高会霞,赵召霞,侯军良,戴二黑..软肝化坚颗粒对肝纤维化模型C57小鼠肝组织α-SMA、TGFβ1表达和Ⅰ型胶原水平的影响[J].临床肝胆病杂志,2012,28(9):685-688,4.基金项目
河北省中医药管理局科学技术研究计划基金项目(2008055) （2008055）
