林产化学与工业2012，Vol.32Issue(4)：113-116,4.
高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚
Simultanous Determination of Quercetin and Kaempferol in Melaleuca alternifolia (Maiden et Betehe) Chee 1 by HPLC
摘要
Abstract
A high-performance liquid chromatography( HPLC) method was developed for simultaneous determination of quercetin and kaempferol in Melaleuca alternifolia ( Maiden et Betehe) Chee 1. The sample was extractd by HCl-MeOH solution(volume ratio 5:100) in water bath, then separated by a C18 column with CH3OH-C2H3N-0. 4% phosphoric acid solution (volume ratio 21 :21 :58) as the mobile phase solution under 370 nm wavelength. The HPLC experiment was performed at room temperature with flow rate 1.0 mL/min. Quercetin and kaempferol contents in M. alternifolia from different area were determined. The result showed that quercetin and kaempferol content in Nanxiao town of Yongning was the highest and that in Yongning was the lowest.关键词
互叶白千层/槲皮素/山奈酚/高效液相色谱法Key words
Melaleuca alternifolia (Maiden et Betehe) Chee 1 /quercetin/kaempferol/HPLC分类
化学化工引用本文复制引用
林霄,董晓敏,陈明生,黄艳,刘布鸣..高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚[J].林产化学与工业,2012,32(4):113-116,4.基金项目
广西自然科学基金资助项目(2010GXNSFB013085) （2010GXNSFB013085）
