林产化学与工业2012，Vol.32Issue(5)：29-33,5.
低能离子注入法诱变选育纤维素酶产生菌的研究
Mutation Breeding of Cellulase Producing Strain by Low Energy Ion Implantation
摘要
Abstract
Trichoderma reesei was used as experimental model,and the effects of low-energy N + implantation on its survival rate,total antioxidant capability (T-AOC),protein concentration and cellulase yield were investigated.The results showed that the dose-survival rate curve took "double saddle shape" with increasing N + implantation doses.It was different from the typical "saddle shape" curve of ion beam implantation.The curve of T-AOC and dose also has two peaks at the doses of 1.50 × 1016 and 2.50 × 1016 cm-2,which were exactly the same as that of the survival rate curve.So it was speculated that the changes of T-AOC probably decided the microbe survival situation after being implanted by low-energy N +.Three cellulase high yielding strains T.reesei 150-1、150-2 and 250-6 were obtained and the average production of cellulase was raised by 24.3％ in shaking flasks.comparing with the control strain,the mutants were potent in accumulating cellulase especially in the later stage.关键词
里氏木霉/纤维素酶/氮离子注入/"双马鞍型"曲线Key words
Trichoderma reesei /cellulose/N + implantation /" double saddle shape" curve分类
化学化工引用本文复制引用
张宁,蒋剑春,杨静,卫民,赵剑,童娅娟..低能离子注入法诱变选育纤维素酶产生菌的研究[J].林产化学与工业,2012,32(5):29-33,5.基金项目
国家自然科学基金资助项目(31100429) （31100429）
