临床神经病学杂志2012，Vol.25Issue(4)：282-284,3.
2-(2-苯并呋喃基)-2-咪唑啉对实验性自身免疫性脑脊髓炎小鼠脊髓胶质纤维酸性蛋白表达的影响
Effects of 2-(2-benzofuranyl)-2-imidazoline on the expression of glial fibrillary acidic portein in spinal cord of mice with experimental autoimmune encephalomyelitis
摘要
Abstract
Objective To investigate the effect of imidazole Ⅱ class receptor (I2R) high selectivity ligands 2-(2-benzofuranyl)-2-imidazoline (2-BF1) on the expression of glial fibrillary acidic portein (GFAP) in spinal cord of mice with experimental autoimmune encephalomyelitis (EAE)- Methods Thirty C57BL/6 mice were divided into EAE group, 2-BF1 group and control group, 10 mice in each group. The EAE and 2-BF1 groups were inducted to be EAE models by subcutaneous injected antigen. And 2-BFI was intraperitoneally injected in 2-BF1 group at same day for 14 d. Then, the score of neurologic impairment was meaeured twice in each group everyday. At 19 d after the modle made, the pathological changes of spinal cord was examinated. The degrees of spinal cord inflammatory cells infiltration and myelin depigmentalion and GFAP expression were observed by immunohistochemislry method. Results The score of neurologic impairment in 2-BFJ group (4. 17 ± 3. 65) was significant lower than EAE group (10. 41 ± 3.02) (P < 0. 01 ) ; the spinal cord pathological score ( 1. 10 ± 0. 59) was significant decreased than EAE group (2.38±0.86)(P<0.01);thequantity of GFAP positive cells [(18.83 ± 2. 31 )/HP] was significantly more than EAE group[(12.25 ±2.66)/HP ](P<0.01) . Conclusion 2-BFI can redunce the EAE development, which maybe related to pormote GFAP expression in central nervous system.关键词
2-(2-苯并呋喃基)-2-咪唑啉/实验性自身免疫性脑脊髓炎/星形胶质细胞Key words
2-(2-benzofuranyl)-2-imidazoline/ experimental autoimmune encephalomyelitis/ astrocyte分类
医药卫生引用本文复制引用
厉芳,郑荣远,朱振国,张正学,刘银凤,张元涛..2-(2-苯并呋喃基)-2-咪唑啉对实验性自身免疫性脑脊髓炎小鼠脊髓胶质纤维酸性蛋白表达的影响[J].临床神经病学杂志,2012,25(4):282-284,3.基金项目
国家自然科学基金资助项目(81070960) （81070960）
浙江省自然科学基金资助项目(Y2074941) （Y2074941）
温州市科技局资助项目(Y20090285) （Y20090285）
