环境与职业医学2012，Vol.29Issue(9)：583-585,588,4.
二巯基丙磺酸钠对氯化汞中毒大鼠肾脏基因表达的影响
Effect of Sodium Dimercaptopropanesulfonate on Gene Expression of Kidney in Mercuric Chloride Treated Rats
摘要
Abstract
[ Objective ] To explore the effect of sodium dimercaptopropanesulfonate (DMPS) on gene expression of kidney in HgCl2 treated rats by gene chips technology, and to provide theoretical basis for the molecular machinery of inorganic mercury-induced kidney injury and treatment. [ Methods ] Thirty SD male rats were randomly divided into 3 groups (10 each group). An animal model of kidney injury was established by subcutaneous HgCl2 injection into the positive control group and the treatment group, and the negative control group was given 0.9% NaCl. Then the treatment group were intramuscularly injected with DMPS, the negative and the positive control groups were injected with 0.9% NaCl. After treatment, differentially expressed genes were identified by gene chips and validated by reverse transcription-polymerase chain reaction (RT-PCR). [ Results ] A total of 385 genes were identified differentially expressed between the negative and the positive control groups; 183 between the negative control group and the treatment group; and 223 between the positive control group and the treatment group. Among the 23 genes expressed differentially involved in DMPS treatment, the functions included xenobiotic metabolism, oxidative stress response, inflammatory response, ion transport, signal transduction, transcription regulation, cell proliferation and apoptosis, collagen fibril organization and cartilage development, neurotransmitter metabolism, and lipid, glucose and amino acid metabolism, etc. Selected results were confirmed by RT-PCR. [ Conclusion ] DMPS can recover the differentially expressed genes of kidney induced by HgCl2, and these genes may possibly play an important role in kidney injury by mercury exposure and in subsequent DMPS treatment.关键词
二巯基丙磺酸钠/氯化汞/肾脏损伤/基因芯片Key words
sodium dimercaptopropanesulfonate/ mercuric chloride/ kidney injury/ gene chips分类
医药卫生引用本文复制引用
路文芳,王秀琴,姜霞,武晓燕,林海鹏,田宇,刘占旗..二巯基丙磺酸钠对氯化汞中毒大鼠肾脏基因表达的影响[J].环境与职业医学,2012,29(9):583-585,588,4.基金项目
环保公益性行业科研项目(编号:200909101,201009044) （编号:200909101,201009044）
