林业科学2012，Vol.48Issue(8)：87-93,7.
钙对酸化处理平邑甜茶根系抗氧化酶活性及线粒体功能的影响
Effect of Calcium on the Functions of Antioxidant Systems and Mitochondria in Malus hupehensis var.pingyiensis Roots under Acid Treatment
摘要
Abstract
The purpose of this paper is to explore the effects of acid treatment and exogenous application of calcium to nutrient solution on the functions of antioxidant systems and mitochondria in Malus hupehensis var. pingyiensis roots. The M. hupehensis var. pingyiensis rootstock seedlings were cultured in 1/2 Hoagland nutrient solutions of different pH (3,4, 5 and 6) and different Ca2+ concentrations (10, 20 and 30 mmol·L-1) with pH3 , respectively. The antioxidant enzyme activities and the characteristics of mitochondria were measured in day 0,2,3 and 4. Compared with the control (pH6) , the maximum activity of SOD, CAT in the treatments occurred in day 2, and the change of POD activity was different, the maximum activity of POD occurred in day 2 in pH 3 and pH 4, and in day 3 pH 5. Meanwhile, the mitochondrial membrane permeability, potential ( △ψm ) and cytochrome c/a decreased with the increasing of the acidity and the treatment time and the content of H2O2 was decreased at first and then increased. The exogenous application of calcium (10 and 20 mmol·L-1) in the nutrient solution under acid treatment ( pH 3) increased the activities of SOD, POD and CAT, relieved the damage of the mitochondrial functions by the acidity and inhibited the accumulation of H2O2 (the decline, with 10 and 20 mmol·L-1, was 6.78% and 13.43% of the accumulation caused by the single acid treatment, respectively). The 20 mmol·L-1 Ca2+ had better effect whereas the 30 mmol·L-1Ca2+ increased the damage and increased the content of H2O2 by 8. 96% . The above results suggested that the acid treatments would damage the roots of M. hupehensis var. pingyiensis and the exogenous application of dilute calcium in the nutrient solution could relieve the damage of acid treatment to the roots.关键词
平邑甜茶/酸化处理/钙/抗氧化系统/线粒体Key words
Malus hupehensis var. pingyiensis / acid treatment/ calcium / antioxidant systems / mitochondria分类
农业科技引用本文复制引用
张召,梁元存,王利,杨洪强,李龙,孙崟崟,谢永波..钙对酸化处理平邑甜茶根系抗氧化酶活性及线粒体功能的影响[J].林业科学,2012,48(8):87-93,7.基金项目
山东农业大学博士后项目资助. （）
