棉花学报2012,Vol.24Issue(4):293-302,10.
陆地棉矮化突变体 Ari1327的矮化机理研究
Study on the Dwarf Mechanism of an Upland Cotton (G.hirsutum L.) Dwarf Mutant,Ari1327
摘要
Abstract
Plant height is an important agronomic trait in upland cotton. Aril327 is a dwarf mutant derived from an American upland cotton variety, Ari971, by 60Co -y-ray irradiation. Aril327 exhibits the dwarf trait during the germination and cotyledon periods. During the budding period, the plant height, internode number and internode length of Ari 1327 were significantly(P < 0.01) smaller or fewer than in the wild type. Therefore, the mutant dwarfism was due to the decrease of average internode length. The longitudinal cell length of mutant internodes was significantly shorter than in the wild type(P < 0.01). The difference in transverse cell diameter between the mutant and the wild type was not significant. Thus, the shorter internodes were induced by inhibition of internode cells. The IAA, GA3 and ABA content of Aril327 was higher than in the wild type. The height of Ari 1327 plants could be restored to wild type levels by exogenous GA3 application. Exogenous BR and IAA had no effect on Aril327. Due to over-expression of genes in the IAA synthetic pathway, the IAA content in Aril327 was remarkably increased. High-density IAA inhibits plant growth; thus, the plant exhibits a dwarf phenotype.关键词
棉花(G.hirsutum L.)/矮化/机理Key words
cotton (G.hirsutum L.)/ dwarfism/ mechanism分类
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王新坤,孙君灵,潘兆娥,张超,何守朴,贾银华,周忠丽,唐灿明,杜雄明..陆地棉矮化突变体 Ari1327的矮化机理研究[J].棉花学报,2012,24(4):293-302,10.基金项目
国家重点基础研究发展计划(973计划)项目(2004CB117306) (973计划)