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陆地棉矮化突变体 Ari1327的矮化机理研究

王新坤 孙君灵 潘兆娥 张超 何守朴 贾银华 周忠丽 唐灿明 杜雄明

棉花学报2012,Vol.24Issue(4):293-302,10.
棉花学报2012,Vol.24Issue(4):293-302,10.

陆地棉矮化突变体 Ari1327的矮化机理研究

Study on the Dwarf Mechanism of an Upland Cotton (G.hirsutum L.) Dwarf Mutant,Ari1327

王新坤 1孙君灵 2潘兆娥 1张超 1何守朴 3贾银华 1周忠丽 1唐灿明 1杜雄明2

作者信息

  • 1. 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南 安阳 455000
  • 2. 南京农业大学,江苏 南京 210095
  • 3. 四川省农业科学院,四川 简阳 641400
  • 折叠

摘要

Abstract

Plant height is an important agronomic trait in upland cotton. Aril327 is a dwarf mutant derived from an American upland cotton variety, Ari971, by 60Co -y-ray irradiation. Aril327 exhibits the dwarf trait during the germination and cotyledon periods. During the budding period, the plant height, internode number and internode length of Ari 1327 were significantly(P < 0.01) smaller or fewer than in the wild type. Therefore, the mutant dwarfism was due to the decrease of average internode length. The longitudinal cell length of mutant internodes was significantly shorter than in the wild type(P < 0.01). The difference in transverse cell diameter between the mutant and the wild type was not significant. Thus, the shorter internodes were induced by inhibition of internode cells. The IAA, GA3 and ABA content of Aril327 was higher than in the wild type. The height of Ari 1327 plants could be restored to wild type levels by exogenous GA3 application. Exogenous BR and IAA had no effect on Aril327. Due to over-expression of genes in the IAA synthetic pathway, the IAA content in Aril327 was remarkably increased. High-density IAA inhibits plant growth; thus, the plant exhibits a dwarf phenotype.

关键词

棉花(G.hirsutum L.)/矮化/机理

Key words

cotton (G.hirsutum L.)/ dwarfism/ mechanism

分类

农业科技

引用本文复制引用

王新坤,孙君灵,潘兆娥,张超,何守朴,贾银华,周忠丽,唐灿明,杜雄明..陆地棉矮化突变体 Ari1327的矮化机理研究[J].棉花学报,2012,24(4):293-302,10.

基金项目

国家重点基础研究发展计划(973计划)项目(2004CB117306) (973计划)

棉花学报

OA北大核心CSCDCSTPCD

1002-7807

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