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一株棉花黄萎病拮抗芽孢细菌的分离鉴定及活性检测

张冬冬 李术娜 郭晓军 朱宝成

棉花学报2012,Vol.24Issue(4):358-362,5.
棉花学报2012,Vol.24Issue(4):358-362,5.

一株棉花黄萎病拮抗芽孢细菌的分离鉴定及活性检测

Isolation, Identification and Activity of an Antagonistic Spore Bacterium against Cotton Verticillium Wilt

张冬冬 1李术娜 1郭晓军 1朱宝成1

作者信息

  • 1. 河北农业大学生命科学学院,河北 保定 071001
  • 折叠

摘要

Abstract

A bacterial strain called Z-5 that had strong inhibition capability against Verticillium dahliae V-190 was obtained via preliminary and secondary screening with the improved agar plate diffusion method. Characterized by morphological and cultural features observation, physiological and biochemical experiments, and 16S rDNA sequence analysis, the Z-5 strain was initially identified as Bacillus malacitensis. Control of cotton Verticillium wilt by this antagonistic bacterium was assessed via potted plants. Results showed that the control efficacy of the antagonistic Z-5 strain on cotton Verticillium wilt was 76.05%. Preliminary results demonstrated that the antagonistic Z-5 bacterial strain could significantly inhibit the growth of V. dahliae.

关键词

棉花黄萎病/大丽轮枝菌/拮抗作用/芽孢细菌/防治效果

Key words

cotton Verticillium wilt/ Verticillium dahliae/ antagonistic activity/ spore bacterium/ efficacy of control

分类

农业科技

引用本文复制引用

张冬冬,李术娜,郭晓军,朱宝成..一株棉花黄萎病拮抗芽孢细菌的分离鉴定及活性检测[J].棉花学报,2012,24(4):358-362,5.

基金项目

保定市科学研究与发展计划(10ZC004) (10ZC004)

棉花学报

OA北大核心CSCDCSTPCD

1002-7807

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