南京农业大学学报2012，Vol.35Issue(5)：103-112,10.
微卫星分子标记在昆虫分子生态学研究上的应用
The application of microsatellite markers in insect molecular ecology
摘要
Abstract
Microsatellites, also known as simple sequence repeats ( SSR) , are composed of small motifs of 1 -6 nucleotides repeated in tandem. They have been widely used in entomological studies in recent years thanks to their characteristics of high level of polymorphism,codominant inheritance,high frequency of occurrence,ease of detection by PCR and low DNA quality required. The aim of the present paper is to review the various methods of microsatellites isolation used in recent years,and the application of microsatellites in molecular ecology studies of insects. We also discussed the drawback of the microsatellite and resolving methods in studies. Finally, the future of the molecular marker was also prospected.关键词
微卫星/微卫星开发/分子标记/昆虫分子生态学Key words
microsatellites/ microsatellites isolation/ molecular marker/insect molecular ecology分类
生物科学引用本文复制引用
孙荆涛,杨现明,葛成,洪晓月..微卫星分子标记在昆虫分子生态学研究上的应用[J].南京农业大学学报,2012,35(5):103-112,10.基金项目
国家公益性行业(农业)科研专项(200903051,200803003) （农业）
教育部博士点基金优先发展领域项目(20110097130005) （20110097130005）
